Section 1: Basic Characterization
Section 2: Pluripotency and the Undifferentiated State
Section 3: Genomic Characterization
Section 4: Stem Cell-based Model Systems
Appendix 1: Recommended Standard Characterization of Stem Cells
Appendix 2: Nomenclature Criteria
Appendix 3: Cell Culture Hygiene Practices
Appendix 5: Assessment of Methods for Genetic Analysis
Appendix 6: Reporting Practices for Publishing Results with Human Pluripotent and Tissue Stem
Acknowledgements
Steering Committee
Tenneille Ludwig, Co-Chair
WiCell Research Institute (USA)
Peter Andrews, Co-Chair
The University of Sheffield (UK)
Ivana Barbaric
The University of Sheffield (UK)
Nissim Benvenisty
Hebrew University (Israel)
Madeline Lancaster
MRC Laboratory of Molecular Biology (UK)
Christine Mummery
Leiden University Medical Center (Netherlands)
Martin Pera
The Jackson Laboratory (USA)
Yoji Sato
National Institute of Health Sciences (Japan)
Glyn Stacey
International Stem Cell Banking Initiative (UK)
Christine A. Wells
University of Melbourne (Australia)
Tongbiao Zhao
Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, Beijing (China)
Working Group Members
Basic Characterization Standards
Glyn Stacey, Co-Chair
International Stem Cell Banking Initiative (UK)
Tenneille Ludwig, Co-Chair
WiCell Research Institute (USA)
Jeremy Crook
The University of Sydney, Chris O’Brien Lifehouse, The University of Wollongong (Australia)
Laurence Daheron
Harvard Stem Cell Institute (USA)
Jon Draper
Stem Cell Network (Canada)
Lyn Healy
Francis Crick Institute (UK)
Andreas Kurtz
Fraunhofer Institute for Biomedical Engineering, Berlin Institute of Health at Charité, (Germany)
Undifferentiated Stem Cells and Pluripotency
Peter Andrews, Co-Chair
The University of Sheffield (UK)
Martin Pera, Co-Chair
The Jackson Laboratory (USA)
Lyn Healy
Francis Crick Institute (UK)
Maneesha Inamdar
Jawaharlal Nehru Centre for Advanced Scientific Research, Institute for Stem Cell Science and Regenerative Medicine (India)
Tenneille Ludwig
WiCell Research Institute (USA)
Noriko Shimasaki
Center for iPS Research and Application, Kyoto, Kyoto Prefectural University of Medicine, Nagoya University, Japan, National University of Singapore, Singapore (Japan)
Austin Smith
University of Exeter (UK)
Genomic Characterization
Ivana Barbaric, Co-Chair
The University of Sheffield (UK)
Tenneille Ludwig, Co-Chair
WiCell Research Institute (USA)
Peter Andrews
The University of Sheffield (UK)
Nissim Benvenisty
Hebrew University (Israel)
Martin Pera
The Jackson Laboratory (USA)
Yoji Sato
National Institute of Health Sciences (Japan)
Claudia Spits
Vrije Universiteit Brussel (Belgium)
Tongbiao Zhao
Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, Beijing (China)
Stem Cell-derived Model Systems (Organoids, Microphysiological Systems, Engineered Cells, Disease Modelling)
Madeline Lancaster, Co-Chair
MRC Laboratory of Molecular Biology (UK)
Christine Mummery, Co-Chair
Leiden University Medical Center (Netherlands)
Anita Bhattacharyya
University of Wisconsin-Madison (USA)
Jeremy Crook
The University of Sydney, Chris O’Brien Lifehouse, The University of Wollongong, NSW, Australia (Australia)
Meritxell Huch
Max Planck Institute of Molecular Cell Biology and Genetics, and Center for Systems Biology, Dresden (Germany)
Kim Jensen
Novo Nordisk Foundation Center for Stem Cell Medicine, University of Copenhagen (Denmark)
Prisca Liberali
Friedrich Miescher Institute for Biomedical Research (Switzerland)
Matthias Lutolf
Swiss Federal Institute of Technology (EPFL) (Switzerland)
Jihwan Song
CHA University, and iPS Bio, Inc. (Korea)
Christine A. Wells
University of Melbourne (Australia)
Tongbiao Zhao
Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine (China)
Supported by ISSCR Staff
Tyler Lamb, Director of Policy
Jack Mosher, Scientific Advisor
Special Acknowledgements
The standards task force would like to thank the numerous reviewers who provided their time and expertise to review the initial draft of the document. We would also like to thank Eric Anthony for his work in establishing this initiative and Saliha Moore for administrative support.
Special thanks to Burroughs Wellcome Fund (BWF grant #1022889), Doris Duke Charitable Foundation (DDCF grant #2022145) (now the Doris Duke Foundation), and the Simons Foundation Autism Research Initiative (SFARI grant #1025472) whose financial contributions made this work possible, and to the California Institute for Regenerative Medicine (CIRM #EDUC1-13553) for supporting the 2022 community engagement workshop.
The ISSCR's Standards for Human Stem Cell Use in Research are strictly copyrighted by the society. No part of this document may be produced in any form without written permission of The International Society for Stem Cell Research. Contact isscr@isscr.org for more information.
©2023 by The International Society for Stem Cell Research. All rights reserved.